Optimization : Extracellular Affinity Maturation by Yeast Display
发布时间：
2023-04-16 21:15
来源：
In vitro affinity maturation of nanobodies and scFv.
We use a combination of mutagenesis and yeast display to greatly improve the binding affinity of your nanobody or scFv. A mutant VHH or scFv library (2-5 x 106) derived from the parental sequence is generated by introducing mutations either across the entire length of the antibody sequence or restricted to the CDR regions and cloned into our proprietary vector for yeast surface expression.
Yeast expressing the mutant library is subjected to several rounds of cell sorting and the concentration of antigen is gradually decreased to select nanobodies with the highest binding affinities. Our yeast display platform controls for the antigen-antibody equilibrium, which is a major advantage compared to phage display.
Key benefits
● Cost-effective and quick optimization method
● No drift-effect like in phage display
● Apparent kD measurements
