Nanobodies for Cell Surface Antigens
发布时间：
2023-04-16 21:14
来源：
Nanobodies are selected against membrane proteins or cell-surface antigens under native conditions.
The VHH candidates are directly selected on living cells by phage display. In this process, our proprietary library is incubated with the antigen expressed on cells. Subsequently, a washing step with antigen-free cells is performed and 3 to 4 rounds of selection follow.
Selected nanobodies are validated by immunofluorescence (IF) or phage-FACS assays. This selection strategy works very well for microbial antigens, such as expressed by parasites, bacteria or viruses.
Key benefits
● Variety of conformational nanobodies against a cell-surface target
