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    MBP-VHH-rFc

    零售价

    ¥ 280

    品牌: 南京瑞源生物 名称: MBP-VHH-rFc 货号: RY-K9003 品类: 蛋白制品

    产品货号: RY-K9003

    • 规格
      • 400ul
      • 50ul
    数量
    -
    +

    库存剩余

    1998

    隐藏域元素占位

    产品描述 说明书

    ▼ 产品规格

    产品名称

     货号  规格

    MBP-VHH-rFc

    RY-K9003-50ul

    50ul

    RY-K9003-400ul

    400ul

     

    Background:

    Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by Western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors.

     

    Specificity:

    Detect MBP and MBP fusion protein.

     

    Storage:

    Store at -20℃. Stable for one year from the date of shipment.

     

    Application:

    WB 1:200-1:2000

     

    Secondary Antibodies:

    Use an anti-Rabbit secondary antibody to detect the VHH antibody.

    声明:本公司出售产品只能用于科研目的,不得用于诊断或者治疗!